领导团队 - 技术研发及设计团队

个人履历

1972年在日本东京大学完成理化学专业博士课程，同年进入日立制作所中央研究所，1994年任主管研究长，2000年任首席科学家，2003年授予Fellow称号，2015年起担任现职。

在中央研究所期间，致力于推进大气压电离质谱分析仪(APIMS)、电场离子化、矩阵SIMS、LC/MS(液相色谱质谱联用仪)等研究，为生物材料电离新技术的发展做出了贡献。特别是矩阵SIMS率先推动了基质辅助电离，并在后来的MALDI-TOF MS中得到应用。从1982年起推动领先世界的荧光DNA测序仪开发工作，推进了日本首例荧光式DNA测序仪的开发和商用。随后，开发了一种高通量的毛细管凝胶阵列DNA测序仪，使人类基因组计划得以早日完成。对科技的重大贡献使其在《科学》的基因组特刊中被称为“幕后英雄”，并被《商业周刊》誉为“亚洲之星”。其主导研发的仪器已作为医疗生物界的基本设备，在世界范围内有了20年以上的广泛使用。

神原博士还进行了基因图谱分析法、基因变异测量技术、以SNPs为主的DNA变异分析方法、和使用磁珠的探针阵列技术等的开发工作。自2006年起，他作为日本文部科学省特定领域研究项目的代表，在单细胞分析技术方面进行了开创性的研究，并持续主持该领域的众多国际会议。

2015年从日立退休后，神原博士出任了公司名誉Fellow，并继续推进积极的技术理念探索。因为认识到生物组织的位置特性分析在组织机能分析中的重要作用，神原博士开创性地推进了生物显微切片采集设备的开发和应用,并力求使用新型工具打造出类似于谷歌地球生命版的生物信息系统。

神原博士在论文、专利领域成果众多（原创论文127篇、科学文章等54篇、专利申请300余项、专利授权100余项）。

自2002年起持续主持”Int Forum on Post Genome Technology”, 自2006年起持续主持”Int. Workshop on Approaches to Single Cell Analysis”，自2014年起持续主持”Africa Int. Biotechnology & Biomedical Conf.”等众多的国际学术会议。

1. * Ministry of Education, Culture, Sports, Science and Technology, Japan

相关链接

• Genome analysis
• https://www.hitachi.com/rd/research/healthcare/genome/ds_slabgel/index.html
• Capillary array DNA sequencer awarded Okochi Memorial Prize
• Hitachi and Perkin-Elmer announce strategic partnership to develop and manufacture new genetic analysis systems

Research interests

• Atmospheric pressure ionization mass spectrometry: Mass spectrometry using ionization at atmospheric pressure to efficiently produce molecular ions. (Holds the patent for this method.)
• Field desorption ionization and collision induced decomposition mass spectrometer for biological molecules.
• Molecular secondary ion mass spectrometry: molecular ion production and mass spectrometry by ion bombardment ionization for non-volatile bio compounds. (1983 IR100)
• Combined system of liquid chromatograph and mass spectrometer. (1994 R&D 100)
• Fluorescent DNA sequencer
• Capillary array DNA sequencer. (Holds many patents on DNA detection systems)
• Technologies for analyzing gene expressions as well as DNA diagnostics.
• Technologies and instruments for single cell and tissue analysis.

Construction of an information system just like Google Earth for the human body.

Major research activity

• 1982-present Development of DNA analysis technology
  Development of the DNA sequencer, gene expression profile analysis method, gene mutation measurement technology, capillary array DNA analysis equipment, SNPs-oriented DNA mutation analysis methods, probe array method using beads, etc.
• 1973-1989 Development of ionization technology for biological samples
  Development of Atmospheric Pressure Ionization Mass Spectrometry, Field Ionization, Molecular-SIMS, LC/MS (Liquid Chromatography/Mass Spectrometer combined device), etc.

Major publications

1. T.Yoda, M.Hosokawa, K.Takahashi, C.Sakanashi, H.Takeyama and H.Kambara, “Site-specific gene expression analysis using an automated tissue micro-dissection punching system,” Scientific Reports 7: 4325(2017).
2. T.Kajiyama, A.Fujii, K.Arikawa, T.Habu, N.Mochizuki, A.Nagatani and H.Kambara, ”Position-Specific Gene Expression Analysis Using a Microgram Dissection Method Combined with On-Bead cDNA Library Construction,” Plant & Cell Physiology 56 (2015): pp. 1320-1328.
3. H.Matsunaga, M.Goto, K.Arikawa, M.Shirai, H.Tsunoda, H.Huang and H.Kambara, “A highly sensitive and accurate gene expression analysis by sequencing (“bead-seq”) for a single cell,” Anal. Biochem 471, pp. 9-16 (2015).
4. K.Taniguchi, T.Kajiyama and H.Kambara, “Quantitative analysis of gene expression in a single-cell by qPCR,” Nature Method 6, pp. 503-506(2009).
5. G.Zhou, M.Kamahori, K.Okano, K.Harada and H.Kambara, ”Miniaturized pyrosequencer for DNA analysis with capillaries to deliver deoxynucleotides,” Electrophoresis 22(2001): pp. 3497-3504.
6. G.Zhou, M.Kamahori, K.Okano, G.Chuan, K.Harada, and H.Kambara,”Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER),” Nucleic Acid Res. 29(2001): No. 19 e93.
7. Y.Kohara, H.Noda, K.Okano and H.Kambara, ”DNA hybridization using “Bead-Array”: Probe-attached beads arrayed in a capillary in a predetermined order” Nucleic Acid Res. suppl 1 (2001): pp. 83-84.
8. T. Anazawa, S.Takahashi and H.Kambara, “A capillary array gel electrophoresis system using multiple laser focusing for DNA sequencing,” Anal. Chem. 68 (1996): pp. 3699-2704.
9. H.Kambara and S.Takahashi, “Multiple-sheath flow capillary array DNA analyser”, Nature 361, pp. 565-566 (1993).
10. U.S. Patent US6224733 B1, Japanese Patent P2974495（Priority claim date: 24th April 1992: DNA detector and DNA detection method, by S. Takahashi and H. Kambara.
11. M.Sakairi and H.Kambara, “Atmospheric Pressure Spray Ionization for Liquid Chromatography/Mass spectrometry,” Anal. Chem. 61 (1989): pp. 1159-1164.
12. H.Kambara, T.Nishikawa, Y.Katayama and T.Yamaguchi, ”Optimization of parameters in a SNA sequenator using fluorescence detection” Bio/Technology 6, 816-821(1988).
13. Japanese Patent P2013548 (Priority claim date: 1st February 1984) JP1994043135: Device for determining base sequence of nucleic acids, by H. Kambara, O. Okada, et al. (9th February 1994 Patent Application Notification No. 特公平6-10665; 1st February 1984 Patent Application No.特願昭59-15226)
14. M.Sakairi and H.Kambara, “Characteristics of a Liquid Chromatograph/Atmospheric Pressure Ionization Mass Spectrometer”, Anal Chem 60 (1988): pp. 774-780.
15. K.Harada, M.Suzuki and H.Kambara, “Diethanolamine Assisted Secondary Ion Mass Spectrometry of Naturally Occurring Complex Oligosaccharides,” Org. Mass Spectrom. 17 (1982): pp. 386-391.
16. H.Kambara and S.Hishida, “Secondary Ion Mass Spectra of Nonvolatile Bioorganic Compounds,” Anal Chem. 53 (1981): pp. 2340-2344.
17. H.Kambara and I.Kanomata, “Determination of Impurities in Gases by Atmospheric pressure Ionization Mass Spectrometry,” Anal. Chem. 49 (1977): pp. 276-275.

Kambara has received numerous recognitions for his contributions to the field of analytical instruments, in particular for his contributions to the development of the capillary array DNA sequencer.

Awards & Recognitions

2019.03

Order of the Rising Sun. Gold Rays with Rosette

2009.03

61st (FY2008) CSJ Award for the "development of capillary array DNA sequencer for genome analysis“
(The Chemical Society of Japan)

2004.01

The Asahi Prize for "development of a high-performance DNA sequencer"

2003.05

National Medal of Honor with Purple Ribbon

2002.07

Selected as one of BusinessWeek's "Star of Asia"

2002.03 (joint)

48th Okochi Memorial Grand Technology Prize for "development of the capillary array DNA sequencer”

2001.04

Commendation by the Minister of Education, Culture, Sports, Science & Technology to Persons of Scientific & Technological Research Merits, for "research on fluorescence detection method DNA base sequencing device"

1994.09 (joint)

R&D Magazine, R&D100 Award for "development of LC/MS system"

1989.04 (joint)

Commendation by the Minister of State for Science & Technology for Noteworthy Inventions, for "side entry laser irradiation method for DNA sequencer"

1983.09 (joint)

Industrial Research & Development IR100 Award for "development of molecular SIMS"

1982.05

Japan Mass Spectrometry Society Award for "new ionization technology“

Education

1972.03

Ph.D., University of Tokyo, Faculty of Science, Chemistry

1969.03

M.Sc., University of Tokyo, Faculty of Science, Chemistry

1967.03

B.Sc., University of Tokyo, Dept. of Pure and Applied Science

Employment & Professional Activities

2017.04 - present

Founder & CEO, Frontier Biosystems, Inc.

2015.04 - present

Emeritus Fellow, Hitachi, Ltd.

2011.04 - present

Distinguished Guest Research Professor, Waseda University

2010.05 - 2015.03

Visiting Professor, Medical School of Nanjing University

2005.07 - 2015.03

Visiting Professor, Graduate School of Frontier Science, University of Tokyo

2003.06 - 2015.03

Fellow, Hitachi Ltd.

2000.04 - 2015.03

Visiting Professor, Tokyo University of Agriculture and Technology

2000.02 - 2003.06

Senior Chief Scientist - Corporate Technology, Hitachi Ltd.

1994.08 - 2000.02

Senior Chief Researcher, Central Research Laboratory, Hitachi Ltd.

1990.02 - 1994.08

Chief Researcher, Central Research Laboratory, Hitachi, Ltd.

1989.08 - 1990.02

Chief Researcher, Advanced Research Laboratory, Hitachi, Ltd.

1985.04 - 1989.08

Senior Researcher, Advanced Research Laboratory, Hitachi, Ltd.

1981.08 - 1985.04

Senior Researcher, Central Research Laboratory, Hitachi, Ltd.

1977.10 - 1979.01

Visiting Researcher at Univ. of California, Berkeley, Space Sciences Lab.

1972.04.01

Joined Central Research Laboratory, Hitachi Ltd.